4/10/2023 0 Comments The isolator![]() Invitrogen™ RNA later™ and Invitrogen™ RNA later™-ICE RNA stabilization solutions provide more flexibility and time to allow the researcher to postpone RNA purification for days, weeks, or even months after tissue collection, without sacrificing the integrity of the RNA. ![]() While this freezing and preprocessing allows the researcher more control over the purification conditions, our experience and feedback from customers confirm that this is a complex, time-consuming, and laborious process. The frozen samples are often preprocessed to select a desired mass or to partially pulverize the sample before exposure to denaturant. A common solution to these problems is to freeze the tissue/cells in liquid nitrogen or on dry ice. This can be problematic when tissues or cells are hard (e.g., bone, roots), when they contain capsules or walls (e.g., yeast, gram-positive bacteria, spores), when workflows prevent processing immediately after collection (e.g., transport from a site of collection to another location for processing), or when samples are numerous (making rapid processing difficult). During sample disruption for RNA isolation, it is crucial that the lytic agent or denaturant be in contact with the cellular contents at the moment that the cells are disrupted. Finding the most appropriate method of cell or tissue disruption for your specific starting material is important for maximizing the yield and quality of your RNA preparation.
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